Audhya Lab
Publications - 2009-Present
44. Cox, N.J., Unlu, G., Bisnett, B.J., Meister, T.R., Condon, B., Luo, P.M., Smith, T.J., Hanna, M., Chhetri, A., Soderblom, E.J., Audhya, A., Knapik, E.W., and Boyce, M. (2017) Dynamic glycosylation governs the vertebrate COPII protein trafficking pathway. Biochemistry. In press.

43. Frankel, E.B., Shankar, R., Moresco, J.J., Yates, J.R., Volkmann, N., and Audhya, A. (2017) Ist1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos. Nat. Commun. 8:1439. Link

42. Hanna, M.G., Block, S., Frankel, E.B., Hou, F., Johnson, A., Yuan, L., Knight, G., Moresco, J.J., Yates, J.R., Ashton, R., Schekman, R., Tong, Y., and Audhya, A. (2017) TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments. Proc. Natl. Acad. Sci. USA. 114: E7707-E7716. Link

41. Frankel, E.B. and Audhya, A. (2017) ESCRT-dependent cargo sorting at multivesicular endosomes. Semin Cell Dev Biol. In press. Link

40. Konig, J., Frankel, E.B., Audhya, A.*, and Muller-Reichert, T.* (2017) Membrane remodeling during embryonic abscission in Caenorhabditis elegans. J. Cell Biol. 216: 1277-1286. (* denotes co-corresponding authors) Link

39. Elsayed, L., Mohammed, I., Hamed, A., Elseed, M., Johnson, A., Mairey, M., Mohamed, H.E., Idris, M., Salih, M., Elsadig, S., Koko, M., Mohamed, A., Raymond, L., Coutelier, M., Darios, F., Siddig, R., Ahmed, A., Babai, A., Malik, H., Omer, Z., Mohamed, E., Eltahir, H., Magboul, N.A., Bushara, E., Elnour, A., Rahim, S.A., Alattaya, A., Elbashir, M., Ibrahim, M., Durr, A., Audhya, A., Ahmed, A., Stevanin, G., and Brice, A. (2017) Hereditary spastic paraplegias: Identification of a novel SPG57 variant affecting TFG oligomerization and description of HSP subtypes in Sudan. Eur. J. Hum. Genet. 25: 100-110. Link

38. Wang, L., Johnson, A., Hanna, M., and Audhya, A. (2016) Eps15 membrane binding and bending activity acts redundantly with Fcho1 during clathrin-mediated endocytosis. Mol. Biol. Cell. 27: 2676-2687. Link

37. Joo, J.H., Wang, B., Frankel, E., Ge, L., Xu, L., Iyengar, R., Li-Harms, X., Wright, C., Shaw, P.I., Lindsten, T., Green, D.R., Peng, J., Hendershot, L., Kilic, F., Sze, J.Y., Audhya, A., and Kundu, M. (2016) Non-Canonical Role of ULK/ATG1 in ER-to-Golgi Trafficking is Essential for Cellular Homeostasis. Mol. Cell. 62: 491-506. Link

36. Frankel, E.B., and Audhya, A. (2016) Burning cellular bridges: Two pathways to the big breakup. J. Cell Biol. 212: 491-493. Link

35. Hanna, M.G., Mela, I., Wang, L., Henderson, R.M., Chapman, E.R., Edwardson, J.M., and Audhya, A. (2016) Sar1 GTPase activity is regulated by membrane curvature. J. Biol. Chem. 291: 1014-1027. Link

34. Callaci, S., Morrison, K., Shao, X., Schuh, A.L., Wang, Y., Yates, J.R. 3rd, Hardin, J., and Audhya, A. (2015) Phosphoregulation of the C. elegans cadherin-catenin complex. Biochem. J. 472: 339-352. Link

33. Spear, J.M., Koborssy, D.A., Schwartz, A.B., Johnson, A.J., Audhya, A., Fadool, D.A., and Stagg, S.M. (2015) Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a. BMC Biochem. 16: 16. Link

32. Li, Z., Venegas, V., Nagaoka, Y., Morino, E., Raghavan, P., Audhya, A., Nakanishi, Y, and Zhou, Z. (2015) Necrotic cells actively attract phagocytes through the collaborative action of two distinct PS-exposure mechanisms. PLoS Genet. 11: e1005285. Link

31. Hwang, B., Phan, F.P., McCool, K., Choi, E.Y., You, J., Johnson, A., Audhya, A., and Miyamoto, S. (2015) Quantification of cellular NEMO content and its impact on NF-kB activation by genotoxic stress. PLoS One. 10: e0116374. Link

30. Schuh, A.L., Hanna, M., Quinney, K., Wang, L., Sarkeshik, A., Yates, J.R., and Audhya, A. (2015) The VPS-20 Subunit of the Endosomal Sorting Complex ESCRT-III Exhibits an Open Conformation in the Absence of Upstream Activation. Biochem J. 466: 625-637. Link

29. Johnson, A., Bhattacharya, N., Hanna, M., Pennington, J.G., Schuh, A.L., Wang, L., Otegui, M.S., Stagg, S.M., and Audhya, A. (2015) TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. EMBO J. 34: 811-827. Link

28. Takahashi, H., Mayers, J.R., Wang, L., Edwardson, J.M., and Audhya, A. (2015) Hrs and STAM function synergistically to bind ubiquitin-modified cargoes in vitro. Biophys. J. 108: 76-84. Link

27. Wan, J., Zhu, F., Zasadil, L., Yu, J., Wang, L., Johnson, A., Berthier, E., Beebe, D.J., Audhya, A., and Weaver, B.A. (2014) A Golgi localized pool of the mitotic checkpoint component Mad1 controls integrin secretion and cell migration. Curr. Biol. 24: 2687-2692. Link

26. Hsu, K.T., Yu, X.M., Audhya, A., Jaume, J.C., Lloyd, R.V., Miyamoto, S., Prolla, T.A., and Chen, H. (2014) Novel Approaches in Anaplastic Thyroid Cancer Therapy. Oncologist. 19: 1148-1155. Link

25. Shen, Q., Schuh, A.L. Zheng, Y., Quinney, K., Wang, L., Hanna, M., Mitchell, J.C., Otegui, M.S., Ahlquist, P., Cui, Q., and Audhya, A. (2014) Mechanisms governing ESCRT-III spiral filament assembly. J. Cell Biol. 206: 763-777. Link

24. Kebede, M., Oler, A., Balloon, A.J., Johnson, A., Rabaglia, M., Stapleton, D., Schueler, K., Floyd, B., Richards, O., Raines, S., Gregg, T., Eliceiri, K., Weisshaar J., Rhodes, C., Thorstenson, C., Keller, M.P., Coon, J., Audhya, A., and Attie, A.D. (2014) Sorcs1 is Required for Normal Dense Core Vesicle Biogenesis in Metabolically Stressed Beta-Cells. J. Clin. Invest. 124: 4240-4256. Link

23. Wang, L., and Audhya, A. (2014) In vivo imaging of C. elegans endocytosis. Methods. 68: 518-528. Link

22. Wickert, L.E., Karta, M.R., Audhya, A., Gern, J.E., and Bertics, P.J. (2014) Simvastatin attenuates rhinovirus-induced interferon and CXCL10 secretion from monocytic cells in vitro. J. Leukoc. Biol. 95: 951-959. Link

21. Schuh, A.L., and Audhya, A. (2014) The ESCRT machinery: From the plasma membrane to endosomes and back again. Crit. Rev. Biochem. Mol. Biol. 49: 242-261. Link

20. Bahmanyar, S., Biggs, R., Schuh, A.L., Desai, A., Müller-Reichert, T., Audhya, A., Dixon, J.E., and Oegema, K. (2014) Spatial control of phospholipid flux restricts endoplasmic reticulum sheet formation to allow nuclear envelope breakdown. Genes Dev. 28: 121-126. Link

19. Green, R., Mayers, J.R., Lewellyn, L., Desai, A., Audhya, A., and Oegema, K. (2013) The midbody ring scaffolds abscission in the absence of midbody microtubules in the C. elegans embryo. J. Cell Biol. 203: 505-520. Link

18. Mayers, J.R., Wang, L., Pramanik, J., Johnson, A., Wang, Y., Sarkeshik, A., Saengsawang, W., Yates, J.R., and Audhya, A. (2013) Regulation of ubiquitin-dependent cargo sorting by multiple endocytic adaptors at the plasma membrane. Proc. Natl. Acad. Sci. USA. 110: 11857-11862. Link

17. Beetz, C., Johnson, A., Schuh, A., Thakur, S., Varga, R., Fothergill, T., Hertel, N., Bomba-Warczak, E., Thiele, H., Nurnberg, G., Altmuller, J., Saxena, R., Chapman, E.R., Dent, E.W., Nurnberg, P., and Audhya, A. (2013) Inhibition of TFG function causes hereditary axon degeneration by impairing ER structure. Proc. Natl. Acad. Sci. USA. 110: 5091-5096. Link

16. Hanna, M., Wang, L., and Audhya, A. (2013) Worming our way in and out of the C. elegans germline and developing embryo. Traffic. 14: 471-478. Link

15. Morrison, K., Witte, K., Mayers, J.R., Schuh, A.L., and Audhya, A. (2012) Role of acidic phospholipids and nucleotides in regulating membrane binding and activity of a calcium-independent phospholipase A2 isoform. J. Biol. Chem. 287: 38824-38834. Link

14. Ryan, S.D., Britigan, E.M., Zasadil, L.M., Witte, K., Audhya, A., Roopra, A., and Weaver, B.A. (2012) Up-regulation of the mitotic checkpoint component Mad1 causes chromosomal instability and resistance to microtubule poisons. Proc. Natl. Acad. Sci. USA. 109: E2205-E2214. Link

13. Ling, Y., Stefan, C.J., MacGurn, J.A., Audhya, A., and Emr, S.D. (2012) The dual PH domain protein Opy1 functions as a sensor and modulator of PtdIns(4,5)P2 synthesis. EMBO J. 31: 2882-2894. Link

12. Joseph-Strauss, D., Gorjanacz, M., Santarella-Mellwig, R., Voronina, E., Audhya, A., and Cohen-Fix, O. (2012) Sm protein down-regulation leads to defects in nuclear pore complex disassembly and distribution in C. elegans embryos. Dev. Biol. 15: 445-457. Link

11. Mayers, J.R., and Audhya, A. (2012) Vesicle formation within endosomes: An ESCRT marks the spot. Commun. Integr. Biol. 5: 50-56. Link

10. Schuh, A.L., and Audhya, A. (2012) Phosphoinositide signaling during membrane transport in Saccharomyces cerevisiae. Subcell. Biochem. 59: 35-63. Link

9. Fyfe, I., # Schuh, A.L., # Edwardson, J.M., and Audhya, A. (2011) Association of the endosomal sorting complex ESCRT-II with the Vps20 subunit of ESCRT-III generates a curvature sensitive protein complex capable of nucleating filaments of ESCRT-III. J. Biol. Chem. 286: 34262-34270. Link (# denotes equal contribution)

8. Park, S., Patterson, E.E., Cobb, J., Audhya, A., Gartenberg, M.R., and Fox, C.A. (2011) Palmitoylation controls the dynamics of budding yeast heterochromatin via the telomere-binding protein Rif1. Proc. Natl. Acad. Sci. USA. 108: 14572-14577. Link

7. Witte, K., Schuh, A.L., Hegermann, J., Sarkeshik, A., Mayers, J.R., Schwarze, K., Yates, J.R., Eimer, S., and Audhya, A. (2011) TFG-1 function in protein secretion and oncogenesis. Nat. Cell Biol. 13: 550-558. Link

6. Green, R., Kao, H.,# Audhya, A.,# Arur, S., Mayers, J.R., Fridolfsson, H., Schulman, M., Schloissnig, S., Niessen, S., Laband, K., Wang, S., Starr, D., Hyman, A., Schedl, T., Desai, A., Piano, F., Gunsalus, K.C., and Oegema, K. (2011) A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Cell. 145: 470-482. Link (# denotes equal contribution)

5. Mayers, J.R., Fyfe, I., Schuh, A.L., Chapman, E.R., Edwardson, J.M., and Audhya, A. (2011) ESCRT-0 assembles as a heterotetrameric complex on membranes and binds multiple ubiquitinylated cargoes simultaneously. J. Biol. Chem. 286: 9636-9645. Link

4. Zaidel-Bar, R., Joyce, M.J., Lynch, A.M., Witte, K., Audhya, A., and Hardin, J. (2010). The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogensis. J. Cell Biol. 191: 761-769. Link

3. Shi, A., Chen, C.C., Banerjee, R., Glodowski, D., Audhya, A., Rongo, C., and Grant, B.D. (2010). EHBP-1 functions with RAB-10 during endocytic recycling in C. elegans. Mol. Biol. Cell. 21: 2930-2943. Link

2. Ziel, J.W., Hagedorn, E.J., Audhya, A., and Sherwood, D.R. (2009). UNC-6 (Netrin) orients the invasive membrane of the anchor cell in C. elegans. Nat. Cell Biol. 11: 183-189. Link

1. Rodenas, E., Klerkx, E.P., Ayuso, C., Audhya, A., and Askjaer, P. (2009). Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly. Dev. Biol. 327: 399-409. Link