Audhya Lab
Regulatory mechanisms that control vesicle biogenesis
Our laboratory is committed to understanding the fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including biochemistry, structural biology, biophysics, genetics, molecular biology and high-resolution fluorescence and electron microscopy.

Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes.
Recent News
October 2017: Elisa Frankel's manuscript titled, "Ist1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos" is accepted for publication in Nature Communications.
August 2017: Elisa Frankel's review titled, "ESCRT-dependent cargo sorting at multivesicular endosomes" is accepted for publication in Seminars in Cell and Developmental Biology.
July 2017: Mike Hanna's manuscript titled, "TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER–Golgi intermediate compartments" is accepted for publication in PNAS.
February 2017: A new collaborative paper entitled, "Membrane remodeling during embryonic abscission in Caenorhabditis elegans" is accepted for publication in the Journal of Cell Biology.
July 2016: Lei Wang's manuscript titled, "Eps15 membrane binding and bending activity acts redundantly with Fcho1 during clathrin-mediated endocytosis" is accepted for publication in Molecular Biology of the Cell.
June 2016: A new collaborative paper entitled, "Hereditary spastic paraplegias: Identification of a novel SPG57 variant affecting TFG oligomerization and description of HSP subtypes in Sudan" is accepted for publication in the European Journal of Human Genetics.
April 2016: A new collaborative paper titled, "The Noncanonical Role of ULK/ATG1 in ER-to-Golgi Trafficking Is Essential for Cellular Homeostasis" is accepted at Molecular Cell.
February 2016: Elisa Frankel's comment entitled, "Burning cellular bridges: Two pathways to the big breakup" is accepted for publication in the Journal of Cell Biology.
November 2015: Michael Hanna's manuscript titled, "Sar1 GTPase Activity Is Regulated by Membrane Curvature" is accepted for publication in the Journal of Biological Chemistry.
October 2015: Sandhya Callaci's manuscript titled, "Phosphoregulation of the C. elegans cadherin-catenin complex" is accepted for publication in the Biochemical Journal.
July 2015: A new collaborative paper titled, "Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a" is accepted at BMC Biochemistry.
June 2015: A new collaborative paper titled, "Necrotic Cells Actively Attract Phagocytes through the Collaborative Action of Two Distinct PS-Exposure Mechanisms" is accepted at PLoS Genetics.
March 2015: A new collaborative paper titled, "Quantification of cellular NEMO content and its impact on NF-κB activation by genotoxic stress" is accepted at PLoS One.
January 2015: Amber Schuh's manuscript titled, "The VPS-20 Subunit of the Endosomal Sorting Complex ESCRT-III Exhibits an Open Conformation in the Absence of Upstream Activation" is accepted and appears online in the Biochemical Journal.
Jon Audhya Lab Group Shot 1